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. 2009 Oct 23;5(10):e1000702. doi: 10.1371/journal.pgen.1000702

Figure 7. HORMAD1 localization in WT oocytes.

Figure 7

SYCP3, HORMAD1 and SYCP1 were detected by IF on nuclear surface spreads of WT oocytes at leptotene (from 16.5 dpc ovaries; A), zygotene (16.5 dpc; B), pachytene (16.5 dpc; C), and diplotene/dictyate (19.5 dpc; D). HORMAD1 preferentially localizes to unsynapsed chromosome axes and is depleted from synapsed axes during zygotene, pachytene and diplotene. HORMAD1 re-accumulates on desynapsing axes in all diplotene stage oocytes (n = 100 cells) and co-localizes with SYCP3 until AEs are disassembled during the dictyate stage (D). Arrows and arrowheads indicate synapsed and unsynapsed axes, respectively. Bars, 10 µm.