Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Protein Expr Purif. 2009 May 18;67(2):139–147. doi: 10.1016/j.pep.2009.05.006

Figure 8. Methodological Synchronization.

Figure 8

An experimental map showing how synchronization of QuikChange-based mutagenesis and protein preparatory procedures allows the production of 20 peptides in a five-week time frame. Mutagenesis daily cycle (top) outlines the number of days in which cloning steps such as QuikChange mutagenesis, transformation, DNA purification and sequencing proceeds. 1:3, 2:3, and 3a:3 represents mutagenesis steps to create first peptide construct B3. Protein daily cycle (bottom) outlines the number of days in which expression, purification, and characterization for each peptide proceeds. The resultant DNA constructs are represented in light gray and purified peptides are represented in dark gray.