Table 1. Peptide Expression, Yield and Chemical Characteristics.
Total peptide fusion expressed is compared to the overall yield of the peptide as a function of purification method used. Peptide characteristics such as charge, isoelectric point (IEP), the number of acidic, basic, polar, and non-polar amino acid residues are also tabulated.
| Peptide | % Peptide in Lysate |
Purification Method |
Potential yield (mg) |
Yield (%) |
Charge | IEP | Acidic | Basic | Polar | Non- polar |
|---|---|---|---|---|---|---|---|---|---|---|
| aPP | 26.1 | Ni Step | 43 | 3.5 | -2.15 | 4.75 | 5 | 3 | 12 | 9 |
| B3 | 11.5 | Ni / IE | 2 | 1.0 | -4.06 | 4.42 | 5 | 1 | 6 | 7 |
| B3DQ | 44.5 | Ni Step | N/A | N/A | -4.06 | 4.49 | 5 | 1 | 6 | 7 |
| B3DE* | 45.0 | Ni Step | 50 | 5.0 | -4.05 | 4.62 | 5 | 1 | 6 | 7 |
| B3DK | 45.9 | Ni / IE | 14 | 0.8 | -2.06 | 5.40 | 4 | 2 | 6 | 7 |
| B3DR | 9.3 | Ni / IE | 5 | 1.4 | -2.06 | 5.40 | 4 | 2 | 6 | 7 |
| B3NQ | 25.4 | Ni / IE | 11 | 1.1 | -1.06 | 6.00 | 2 | 1 | 9 | 7 |
| B3NE | 20.9 | Ni / IE | 28 | 2.3 | -2.06 | 5.33 | 3 | 1 | 8 | 7 |
| B3NK | 11.5 | Ni / IE | 8 | 1.0 | -0.06 | 6.92 | 2 | 2 | 8 | 7 |
| B3NR | 24.5 | Ni Gradient | 20 | 1.7 | -0.06 | 6.92 | 2 | 2 | 8 | 7 |
| B3II* | 45.0 | Ni / IE | 70 | 5.8 | -4.23 | 3.77 | 5 | 1 | 6 | 9 |
| B3IQ | 32.2 | Ni Gradient | 31 | 3.9 | -3.23 | 4.10 | 4 | 1 | 7 | 9 |
| B3IE | 35.9 | Ni Gradient | 32 | 16.0 | -4.23 | 3.98 | 5 | 1 | 6 | 9 |
| B3IK | 36.1 | Ni Gradient | 25 | 2.5 | -2.23 | 4.49 | 4 | 2 | 6 | 9 |
| B3IR | 37.6 | Ni Gradient | 35 | 2.9 | -2.23 | 4.49 | 4 | 2 | 6 | 9 |
| B3IL | 30.8 | Ni Gradient | 40 | 4.0 | -3.23 | 4.10 | 4 | 1 | 6 | 10 |
| 3INQ | 27.0 | Ni Gradient | 30 | 3.0 | -1.24 | 4.53 | 2 | 1 | 9 | 9 |
| 3INE | 36.6 | Ni Gradient | 28 | 2.8 | -2.23 | 4.25 | 3 | 1 | 8 | 9 |
| 3INK | 19.2 | Ni Gradient | 25 | 2.5 | -0.24 | 6.14 | 2 | 2 | 8 | 9 |
| 3INR | 29.7 | Ni Gradient | 33 | 3.3 | -0.24 | 6.14 | 2 | 2 | 8 | 9 |
Percent peptide in raw E. coli lystates was calculated using GeneTools (SynGene).
*
Indicates % peptide calculated was estimated by inspection of the stained SDS-PAGE gels. Ni: Ni-Affinity chromatography. IE: ionic exchange chromatography. Acidic, Basic, Polar and Non-polar represent the number of amino acids in the 27-residue peptide that have the particular chemical characteristic.