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. Author manuscript; available in PMC: 2009 Oct 7.
Published in final edited form as: J Comp Neurol. 2007 Feb 20;500(6):1007–1033. doi: 10.1002/cne.21229

Fig. 2.

Fig. 2

Expression pattern for CXCR4 mRNA expression in neurogenic regions of mouse brain. In situ hybridization was performed with a probe specific for CXCR4, using digoxygenin (DIG) labeling (A–F) and fluorescence in situ hybridization (FISH) (G–I) methods. CXCR4 mRNA was expressed in the olfactory bulb (OB), subventricular zone (SVZ), and dentate gyrus (DG) of 5-week-old mouse brain. A: In the OB, CXCR4 mRNA was expressed in the rostral extent of the olfactory ventricle, as well as in the granule, periglomerular, and mitral cell layers. B: In the SVZ, CXCR4 mRNA expression was observed in cells surrounding the ventricle and in the dorsolateral extension of the SVZ. C: In the DG, CXCR4 mRNA was expressed in the granule cell layer including the subgranular zone (SGZ). D–F: In situ hybridization performed using a CXCR4 sense probe to test the specificity of the probe. G–I: CXCR4 mRNA expression patterns obtained by FISH. Comparison of the two methods shows that in each case the expression pattern was the same, thereby validating this fluorescent method of detection. Scale bars = 100 µm.