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. Author manuscript; available in PMC: 2009 Oct 7.
Published in final edited form as: J Comp Neurol. 2007 Feb 20;500(6):1007–1033. doi: 10.1002/cne.21229

Fig. 8.

Fig. 8

Colocalization of CCR2 and EGFP in neurogenic regions of 5-week-old nestin-EGFP transgenic mouse brain. A–I: FISH was performed using a CCR2 antisense probe in conjunction with immuno-staining with a GFP antibody. A–C: In the olfactory bulb (OB), most of the EGFP-expressing cells also expressed CCR2. D–F: In the subventricular zone (SVZ), virtually all the EGFP-expressing cells coexpressed CCR2. G–I: In the dentate gyrus (DG), EGFP-expressing cells expressed CCR2, CCR2 expression being more apparent in nonradial astrocyte-like cells. J–M: Immunohistochemistry was carried out on free-floating sections obtained from nestin-EGFP mouse brain using CCR2 antibody. In the DG, CCR2-immunoreactive cells (red) colocalized with type 2 nestin-EGFP cells (green). M: The CCR2 expression by nestin-EGFP cells at higher magnification (arrowhead). Scale bars = 100 µm in A–I; 50 µm in J–M.