Table I.
Proteins with enhanced expression in S. griseus B-2682 AFN
Match, peptides identified/masses searched; Seq. cov., sequence coverage; Sec. no., presence of a secretion signal; SCO, S. coelicolor database; SAV, S. avermitilis database; SSP, standard spot number generated by PDQuest; SGR, Streptomyces griseus database. The percentage in parentheses refers to amino acid identity between the predicted SGR and SCO proteins.
| ID | SSP | Accession no. | Protein name | Match | Seq. cov. | Molecular mass | Sequence confirmed by PSD or CID | Sec. no. | ADP-ribosylation consensus | Orthologue in SCO/SAV |
|---|---|---|---|---|---|---|---|---|---|---|
| % | kDa | |||||||||
| 1 | 3002 | SGR1460 | OsmC-like ATP/GTP-binding protein | 4/9 | 24 | 14.5 | 6VAHTNWEGNLIEGK19, 20GVVTFDSSGIGDHPVSWPAR39 | No | No | SCO6531 (83%), SAV1862 (84%) |
| 2 | 0004 | SGR2245 | Superoxide dismutase (SodN) | 11/15 | 32 | 15 | 85YPELHQLINDTLK97 | No | No | SCO5254 (87%), SAV2988 (86%) |
| 3 | 4603 | SGR3109 | Putative aminotransferase | 31/43 | 51 | 39.4 | 2ADIQIPADIKPADGR16, 226HIPEFFSLPTAIDNSLK242 | No | No | SCO4366 (87%), SAV3883 (87%) |
| 4 | 4507 | SGR3109 | Putative aminotransferase | 13/29 | 37 | 39.4 | 2ADIQIPADIKPADGR16, 226HIPEFFSLPTAIDNSLK242 | No | No | SCO4366 (87%), SAV3883 (87%) |
| 5 | 1502 | SGR1498 | Xylose ABC transporter solute-binding protein | 32/34 | 56 | 38.8 | 1–27 | 21LAACG25 | SCO6009 (78%), SAV2247 (77%) | |
| 6 | 2402 | SGR1498 | Xylose ABC transporter solute-binding protein | 22/27 | 39 | 38.8 | 47IGLLLPENQTAR58, 125AKDAGIPVVAFDR137, 254GAGISPLPPVTGQDAELAGVQR275 | 1–27 | 21LAACG25 | SCO6009 (78%), SAV2247 (77%) |
| 7 | 2405 | SGR1498 | Xylose ABC transporter solute-binding protein | 41/49 | 56 | 38.8 | 47IGLLLPENQTAR58, 127DAGIPVVAFDR137, 254GAGISPLPPVTGQDAELAGVQR275 | 1–27 | 21LAACG25 | SCO6009 (78%), SAV2247 (77%) |
| 8 | 2404 | SGR1498 | Xylose ABC transporter solute-binding protein | 31/41 | 44 | 38.8 | 47IGLLLPENQTAR58, 127DAGIPVVAFDR137, 254GAGISPLPPVTGQDAELAGVQR275 | 1–27 | 21LAACG25 | SCO6009 (78%), SAV2247 (77%) |
| 9 | 8202 | SGR5704 | Putative Spo0M-type sporulation control protein | 16/24 | 29 | 28.5 | 38IQGGSVAQQIEGLNVGLQAR57, 192GLHQVELTFVADDR205 | 1–17 | No | SCO1793 (81%), SAV6485 (83%) |
| 10 | 4305 | SGR2237 | Putative arginine/ornithine-binding protein | 34/47 | 69 | 33.5 | 178SIEDLCGKPAAVQR191, 231SGGSVAGVNDYPVAVDLAR249, 286EAVDAIIADGSYQK299 | 1–25 | 29LTACG33 | SCO5260 (58%), SAV2982 (58%) |
| 11 | 6701 | SGR5275 | Maltose-binding protein MalE | 21/45 | 43 | 44.8 | 73YVNVPFGDAQNK84, 87NAAQAGDGAPDVIR100 | 1–29 | 18ATACG22 | SCO2231 (48%), SAV5977 (48%) |
| 12 | 1307 | SGR1737 | Glutamate ABC transporter substrate-binding protein | 33/35 | 55 | 33 | 77ADQPYLGFEDQATK90, 149QVGFAGPYYK158, 197KPEYGASVVELAK209 | 1–22 | 19ATACG23 | SCO5776 (40%), SAV2485 (55%) |
| 13 | 1303 | SGR1737 | Glutamate ABC transporter substrate-binding protein | 35/38 | 56 | 33 | 77ADQPYLGFEDQATK90, 131GQVDYYVGTYTINDER146, 149QVGFAGPYYK158, 197KPEYGASVVELAK209 | 1–22 | 19ATACG23 | SCO5776 (40%), SAV2485 (55%) |
| 14 | 4204 | Manganese-superoxide dismutase SodA | 9/14 | 56 | 22.4 | 115FKSDFAAAAAGR126 | No | No | ||
| 15 | 2702 | SGR5280 | Putative α-amylase | 21/25 | 27 | 60.7 | 295SAVFVDNHDTER306, 406AYVAINHEGSALTR419, 103AAFAAMVNTCHAAGVK118, 229LTNPNVYWK237 | 1–35 | No | SCO7020 (78%), SAV5981 (73%), |
| 16 | 1803 | SGR5280 | Putative α-amylase | 25/46 | 44 | 60.7 | 295SAVFVDNHDTER306, 406AYVAINHEGSALTR419 | 1–35 | No | SCO7020 (78%), SAV5981 (73%) |