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. Author manuscript; available in PMC: 2010 Mar 13.
Published in final edited form as: Circ Res. 2009 Jan 22;104(5):609–618. doi: 10.1161/CIRCRESAHA.108.186064

Figure 4.

Figure 4

Klf4 was required for POVPC-induced type VIII collagen gene expression in rat aortic SMCs. A, VSMCs were transfected with Klf4 siRNA oligonucleotides (siKLF4) or control siRNA (siControl) followed by either POVPC (10 μg/mL) or vehicle treatment for 24 hours. B, VSMCs were isolated from the aorta of Klf4-floxed mice and infected with adenovirus expressing Crerecombinase (Klf4 KO) or control adenovirus. KO and control VSMCs were treated with POVPC or vehicle for 24 hours. Expression of Col8a1 gene was measured by quantitative real-time RT-PCR. #*P<0.05 vs vehicle, **P<0.01 vs vehicle. For Western blot analyses, the conditioned media from rat aortic SMCs after transfection with control siRNA (siControl), siSp1, or siKLF4 and treatment with either POVPC (10 μg/mL) (P) or vehicle for 48 hours was fractionated under denaturing conditions on a 10% polyacrylamide gel (C). Type VIII collagen protein was detected with a guinea pig polyclonal antibody raised against bovine type VIII collagen.