Figure 1.

Affinity precipitation of recombinant p53 deletion constructs with peptides, analyzed by Western blot. Bacterial lysates containing 1.5 μg of the indicated p53 constructs were precipitated with different peptide affinity matrices. p53 in eluates was determined by Western blot analysis with PAb240. Lanes 1 show lysate samples containing 7.5 ng of the respective input p53 construct. The cysteine-blocked matrix was used for the control analyses in lanes 2. The indicated peptide-matrices were used for precipitation in lanes 3–5. Position of molecular mass standards is indicated.