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. 2009 Oct 7;106(42):17864–17869. doi: 10.1073/pnas.0904174106

Fig. 3.

Fig. 3.

Single cleavage with IdeS inhibits ADCC and CDC activity without impacting antigen binding. (A) Concentration-dependent effects of intact mAb4 IgG (blue), its single-cleaved version (scIgG) (red), and the F(ab′)2 derivative (black) in ADCC. MDA-MB-231 were the target cells, and human PBMCs were the effector cells. Error bars correspond to the SD of triplicate determinations. (B) Surface levels of intact mAb4 (blue) and single-cleaved IgG (scIgG) of mAb4 (red) were measured by flow cytometry. The shaded area indicates autofluorescence, and the black line indicates fluorescence obtained with the anti-Fc gamma domain antibody alone. (C) CDC activity against WIL2-S cells using intact mAb5 IgG1 (blue), single-cleaved mAb5 (red), and mAb5 F(ab′)2 (black). Error bars correspond the SD of triplicate determinations. (D) Surface levels of intact mAb5 (blue) and single-cleaved mAb5 (red) were measured by flow cytometry on WIL2-S cells.