Table 3.
Isolate | plcA | plcB | plcC | Comment |
M. bovis | Deleted | Deleted | Deleted | plcABC part of the RD5 region (Figure 3). |
M. bovis BCG | Deleted | Deleted | Deleted | plcABC part of the RD5 region (Figure 3). |
CPHL_A | sSNP A → C at position 435. | + | + | All genes predicted to be fully functional. |
K85 | sSNP A → C at position 435. | + | nsSNP C → T (Thr → Ile) at aa position 302. sSNP G → A at position 1506. |
plcC function possibly impaired. |
GM041182 | sSNP A → C at position 435. | + | + | All genes predicted to be fully functional. |
M. microti | Deleted. | Deleted | 5' 867 bp deleted. | plcABC part of the RD5mic region (Figure 3). |
Oryx bacillus | Deleted | 5' 260 bp deleted. | +‡ | plcAB part of the RD5oryx region (Figure 3). |
Dassie bacillus | Deleted | Deleted | Deleted | plcABC part of the RD5das region (Figure 3). |
T17 | sSNP A → C at position 435. | IS6110 insertion at position 1307. | + | plcB function predicted to be abolished. |
EAS054 | sSNP A → C at position 435. | sSNP G → A at position 1404. | + | All genes predicted to be fully functional. |
T92 | Deleted | Deleted | 5' 194 bp deleted. | Major deletion results in removal of plcA, plcB and 5' region of plcC (Figure 3). |
94_M4241A | Deleted | 5' 793 bp deleted. | sSNP T→ C at position 753. | IS6110-associated recombination event has deleted plcA and 5' region of plcB (Figure 3). |
02_1987 | Deletion of 3' end of plcA and 5' end of plcB creates hybrid plcA/B gene. Fusion point at position 145. Results in frameshift and premature protein termination. |
sSNP T→ C at position 753. | Hybrid plcA/B gene predicted to be non-functional. Part of massive genome rearrangements seen in this isolate (Figure S23). | |
T85 | sSNP G → A at position 705. nsSNP T → A (thr → ala) at position 1336. |
+ | sSNP T → C at position 753. nsSNP G → T (gly → cys) at position 1081. |
plcA and plcC functions possibly impaired. |
KZN 4207 | + | + | + | Total homology to H37Rv. |
KZN 1435 | + | + | + | Total homology to H37Rv. |
KZN 605 | + | + | + | Total homology to H37Rv. |
F11 | + | + | + | Total homology to H37Rv. |
Strain C | T insertion at position 104. Altered reading frame and premature protein termination. | + | + | plcA function predicted to be abolished. |
CDC1551 | + | + | + | Total homology to H37Rv. |
Haarlem | A insertion at position 968. Altered reading frame and premature protein termination. | + | + | plcA function predicted to be impaired. |
H37Rv | + | + | + | Defined as wild type sequence |
H37Ra | + | + | + | Total homology to H37Rv. |
+ indicates complete homology to the H37Rv reference sequence.
‡Deletion mapping studies indicates that the plcC gene of the Oryx bacilli is present [23]. The exact sequence of the gene in this species is unknown however.