Table 4.
Clone | Fluorescence | DNA contenta | Microsatellite locusb | |||||||
II-PLC | III-2 | IV-TB4/7 | VI-TB6/1 | VIII-TB8/9 | IX-TB9/6 | X-TB10/17 | XI-53 | |||
J10 RFP | Red | 0.96 | ab | ab | ab | aa | aac | ab | ab | aa |
J10 GFP | Green | 0.96 | ab | ab | ab | aa | ab | ab | ab | aa |
Fly 1 clone 1 | Red | 1.61 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 1 clone 2 | Red | 1.64 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 1 clone 3 | Red | 1.51 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 1 clone 4 | Red | 1.48 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 1 clone 5 | Red | 1.76 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 1 clone 6 | Red | 1.55 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 1 clone 7 | Red | 1.76 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 1 clone 8 | Red | ND | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 2 clone 1 | Red | 1.77 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 2 clone 2 | Green | 1.05 | ab | ab | ab | aa | ab | ab | ab | aa |
Fly 2 clone 3 | Green | 0.99 | ab | ab | ab | aa | ab | ab | ab | aa |
Fly 2 clone 4 | Green | ND | ab | ab | ab | aa | ab | ab | ab | aa |
Fly 2 clone 5 | Red | 0.97 | ab | ab | ab | aa | ab | bb | ab | aa |
Fly 2 clone 6 | Green | 0.93 | ab | ab | ab | aa | ab | ab | ab | aa |
Fly 2 clone 7 | Red | ND | ab | ab | ab | aa | ab | bb | ab | aa |
a. DNA content relative to T. b. brucei strain 427, arbitrarily assigned a DNA content of 1.00. ND, not done.
b. See Table 4 for information on microsatellite loci.
c. There is an allelic difference between J10 RFP and J10 GFP, presumably generated during derivation of these separate lineages.