Table 1.
Identification of protein spots deemed different between sap from control and P. sojae elicitor or B. japonicum treated plants
| Spot No | Annotation [organism] |
TIGR TA ID |
Number of peptides matched | MASCOT Score |
Observed pI/Theoretical pI |
Observed MW/Theoretical MW (kD) |
Signal peptide |
Response to treatmenta |
| 1, 2, 7, 8 | Trypsin inhibitor A precursor [Glycine max (Soybean)] | TA42304_3847 | 5 | 147 | 5.0/4.6 | 10.9/20.1 | Yes | |
| 3 | Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] | AI736946 | 4 | 144 | 8.5/8.5 | 15.7/29.3 | Likely | |
| 4 | Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] | TA42145_3847 | 10 | 385 | 6.1/5.9 | 27.6/25.3 | Yes | |
| 5 | Gamma-glutamyl hydrolase precursor [Glycine max (Soybean)] | TA43569_3847 | 7 | 482 | 7.9/6.3 | 34.6/35.3 | Yes | Bj-- |
| 6 | CPRD14 protein -- Cinnamoyl alcohol dehydrogenase [Vigna unguiculata (cowpea)] | TA46458_3847 | 10 | 537 | 7.9/5.9 | 34.6/35.8 | No | |
| 9 | Trypsin inhibitor A precursor [Glycine max (Soybean)] | TA42304_3847 | 5 | 247 | 4.5/4.6 | 10.4/20.1 | Yes | |
| 10, 11, 23 | Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] | TA41989_3847 | 13 | 705 | 8.7/8.5 | 27.0/29.3 | Likely | |
| 12 | Acid phosphatase precursor [Phaseolus vulgaris (Kidney bean) (French bean)] | TA42402_3847 | 8 | 392 | 7.0/5.8 | 63.6/49.7 | Yes | Bj-- |
| 13, 14 | Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] | TA42145_3847 | 15 | 1042 | 6.4/5.9 | 30.9/25.3 | Yes | |
| 15 | Xyloglucan endotransglycosylase [Malus domestica (Apple) (Malus sylvestris)] | TA43786_3847 | 5 | 205 | 8.4/6.8 | 32.3/31.9 | No | Bj +++ |
| 16 | Cyclophilin [Kandelia candel] | TA43384_3847 | 3 | 89 | 7.0/8.7 | 15.3/18.3 | No | Bj++ |
| 17 | Cytosolic malate dehydrogenase [Glycine max (Soybean)] | TA43756_3847 | 4 | 173 | 6.8/6.3 | 24.6/35.5 | No | Bj++ |
| 18 | 20S proteasome beta subunit C-1 [Arabidopsis thaliana] | TA46641_3847 | 2 | 87 | 5.1/5.3 | 21.0/22.8 | No | Bj++ |
| 19 | Gamma-glutamyl hydrolase precursor [Glycine max (Soybean)] | TA43569_3847 | 6 | 315 | 7.6/6.1 | 37.8/37.7 | Yes | |
| 20 | Cationic peroxidase 1, putative, expressed [Oryza sativa (japonica cultivar-group)] | TA64044_3847 | 2 | 136 | 7.0/5.34 | 10.9/27.0 | Yes | Bj-- |
| 21, 22 | Beta-amylase [Glycine max (Soybean)] | TA44402_3847 | 4 | 203 | 6.8/5.4 | 24.6/56.2 | No | |
| 24 | Putative globulin/legumin [Arabidopsis thaliana] | TA42556_3847 | 4 | 213 | 4.7/5.8 | 37.8/38.3 | No | |
| 25 | Cationic peroxidase 1 precursor [Arachis hypogaea (Peanut)] | TA59475_3847 | 2 | 134 | 7.0/8.1 | 38.7/31.2 | Yes | Ps++ |
| 26 | Putative subtilisin precursor [Glycine max (Soybean)] | TA41917_3847 | 3 | 275 | 7.3/6.6 | 97.8/80.0 | Yes | Ps++ |
a Increase (+) or decrease (-) of spot intensity in response to B. japonicum or P. sojae elicitor treatment is indicated.