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. 2009 Jul 22;29(29):9361–9369. doi: 10.1523/JNEUROSCI.0737-09.2009

Figure 1.

Figure 1.

A–C, Coronal sections (A, B) and sagittal section (C) from the rat atlas of Paxinos and Watson (2005) showing the extent of the SOM (shaded area); the SOM region included the GiV and medullary gigantocellular reticular nucleus (Gi), nucleus paramedianus, and nucleus raphe obscurus that lies immediately dorsal to GiV. Inset in C shows the FG injection site in the ventral horn of the spinal cord at C8–T1 level. D, Retrogradely labeled (FG-immunoreactive) neurons (brown) within the SOM after spinal ventral horn injections of FG; E, double-labeling of FG-ir neurons (brown) for the presence of VGLUT2 mRNA showed that an average of 61% of FG-immunoreactive neurons also contained VGLUT2 mRNA (black puncta) thus marking them as glutamatergic. F, High power photomicrograph of the boxed region in E. Arrows indicate double-labeled (FG and VGLUT2) neurons in the SOM and arrowheads indicate single-labeled (FG) neurons. Inset in F shows enlarged view of two neurons underneath the asterisk—one single-labeled (FG) and one double-labeled (FG and VGLUT2). GiA, Gigantocellular reticular nucleus, α part; IO, inferior olive; py, pyramidal tract; IRt, intermediate reticular nucleus. Scale bars: D–F, 100 μm.