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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2009 Aug 27;16(10):1021–1025. doi: 10.1038/nsmb.1676

Figure 2. Zcchc11 TUTase activity is Lin28-dependent.

Figure 2

(a) Uridylation assays performed with pre-let-7g in the presence of unlabelled UTP, ATP, CTP or GTP. (b) TUTase assay performed with pre-let-7a with a catalytically inactive Zcchc11 protein. A mutant Zcchc11 protein with two mutated aspartate residues (D1008A, D1010A in the NTr) was used in the uridylation assay. c, Uridylation assay with synthetic pre-miR-21 as a substrate in the uridylation assay as in Fig 1(c).