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. Author manuscript; available in PMC: 2010 Oct 14.

Published in final edited form as: J Am Chem Soc. 2009 Oct 14;131(40):14212–14213. doi: 10.1021/ja906642n

Absorbance spectra of reduced and oxidized IYD under anaerobic conditions. The oxidized flavin of IYD () was fully reduced by addition of an equiv. of dithionite (). Electrons were discharged from this reduced flavin after addition of excess I-Tyr (2 equiv.) ().

Inline graphic — Absorbance spectra of reduced and oxidized IYD under anaerobic conditions. The oxidized flavin of IYD () was fully reduced by addition of an equiv. of dithionite (). Electrons were discharged from this reduced flavin after addition of excess I-Tyr (2 equiv.) ().