. Author manuscript; available in PMC: 2010 Oct 14.

Published in final edited form as: J Am Chem Soc. 2009 Oct 14;131(40):14212–14213. doi: 10.1021/ja906642n

Table 1.

Recognition and Processing of Halotyrosines by IYD.

Substrate	K_D^a (μM)	Oxidation of Reduced IYD	Equivalents of Tyrosine Produced^b	pKa (2-Halophenol)²⁴
I-Tyr	0.09 ± 0.04	Yes	0.96 ± 0.02	8.53
Br-Tyr	0.11 ± 0.03	Yes	0.92 ± 0.01	8.35
Cl-Tyr	0.15 ± 0.02	Yes	0.94 ± 0.02	8.48
F-Tyr	1.3 ± 0.2	No	≤ 0.02 ± 0.03	8.86
Tyr	>140	No	--	10.05

As determined by quenching of flavin fluorescence (See Figure S6). Uncertainties derive from the standard deviation of three or more independent measurements.

Generation of the Tyr product was quantified by reverse-phase C-18 HPLC (Figure S3).