Table 1.
Sequence of the primers used in heminested reactions
| Marker/mutation | Forward primer | Reverse primer | Nested primer |
|---|---|---|---|
| D13S141 | GGAACGAAGACAATCAGGAG | GTCCTCCCGGCCTAGTCTTA | ACCACGGAGCAAAGAACAGA |
| D13S175 | TATTGGATACTTGAATCTGCTG | CCACAGAAAGTTTTTCAGCT | TGCATCACCTCACATAGGTTA |
| D13S633 | ACGCTTTACCCCTGAAATC | ACTCGAACTGCTTTGCTTCT | ACGCTTTACCCCTGAAATC |
| D13S1275 | TATTCTGAAGCATCCCAGTG | ATCACTTGAATAAGAAGCCATTTG | TTGTTGCTTCTTACATCATCCT |
| D13S250 | GAAGACAGGGTTATGCCCA | ACAGGATATCCCCAGCAG | CCTGGATGCTAGAGTATTGAAA |
| D13S232 | TTTGAGCCCTTCCTTTCTGA | GGCACAGAAATAAATGTTGAT | TGCTCACTGCTCTTGTGATT |
| D13S1830 | ATTGCTTGAAGCCAGGAGTT | TTTTGTTTGGTACCAGAAGGATTA | CAGCCTGTGCGAGATAGTGAG |
| D13S292 | CGGGGTATTTTAAAACAGAAA | TTTGACACTTTCCAAGTTGC | TAATGGCGGACCATGC |
| GJB2-AT1 | CCTGTAGTCCTGGCTCCTT | GTACCTTTGCTTCCCAAAAA | CTGGGGGACACAGTAAGACT |
| GJB2-AT2 | GAGAATGCTGTCAATATTGGTG | ATGGCTGGCATAGAAGAAAA | TCTGCATCACCCAATCAATA |
| GJB2-TG1 | AGATGAAGAGGGAGGCAG | GAAAACAGAACAGAGTTAAAAGGA | ACACCTTCCAAAAACCTAACAC |
| GJB2-TG2 | TTCCTCTCTGTCTCCTTTACTT | GAATCACTTGAACCCAGGAG | TTCTTTGACTCTCCATGCTG |
| GJB2-AC1 | CACAGCTCAGGATTTTACCA | CCTTTCTGGTCTAGGCGTC | CCGAAACAGGAGGATTGC |
| GJB2 35 del G | GGTGAGGTTGTGTAAGAGTTGG | AAAATGAAGAGGACGGTGAG | CTCGTGGAGTGTTTGTTCCAC |
| GJB2167del T | TTGATCTCCTCGATGTCCTT | GCTCACCGTCCTCTTCATTT | CTTCTTCTCATGTCTCCGGTA |