Table 6.
Interleukin (IL)-10 secretion by peripheral blood mononuclear cells (PBMC) of diabetic patients, non-diabetic tuberculosis (TB) patients and healthy subjects in response to complex mycobacterial antigens and peptide pools of regions of difference (RD1), RD4, RD6 and RD10.
| Concentration of IL-10 (pg/ml) in PBMC culture supernatants of |
||||||
|---|---|---|---|---|---|---|
| Diabetic TB patients |
Non-diabetic TB patients |
Healthy subjects |
||||
| Antigen | Median (range) | E/C* | Median (range) | E/C | Median (range) | E/C |
| No antigen | 29 (≤ 6·9–837) | ≤ 6·9 (≤ 6·9–666) | ≤ 6·9 (≤ 6·9–297) | |||
| M. bovis BCG | 601 (162–1740) | 20·4 | 169 (≤ 6·9–2427)† | 24·4 | 38 (≤ 6·9–1109)‡ | 5·5 |
| M. tuberculosis | 974 (250–6536) | 33 | 221 (≤ 6·9–4934) | 32 | 330 (≤ 6·9–2186)‡ | 47·8 |
| MT-CF | 157 (≤ 6·9–1063) | 5·3 | 81 (≤ 6·9–430) | 12 | 10 (≤ 6·9–273) | 1·4 |
| MT-CW | 747 (54–10 949) | 25·3 | 104 (≤ 6·9–3390) | 15 | 25 (≤ 6·9–2389)‡ | 3·6 |
| RD1pool | 207 (13–519) | 7 | ≤ 6·9 (≤ 6·9–329)† | 1 | ≤ 6·9 (≤ 6·9–198)‡ | 1 |
| RD4pool | 120 (14–352) | 4 | ≤ 6·9 (≤ 6·9–20)† | 1 | ≤ 6·9 (≤ 6·9–166)‡ | 1 |
| RD6pool | 67 (≤ 6·9–1222) | 2·2 | ≤ 6·9 (≤ 6·9–21)† | 1 | ≤ 6·9 (≤ 6·9–62)‡ | 1 |
| RD10pool | 167 (≤ 6·9–561) | 5·6 | ≤ 6·9 (≤ 6·9–14)† | 1 | ≤ 6·9 (≤ 6·9–116)‡ | 1 |
*
E/C: cytokine concentration in antigen stimulated cultures/cytokine concentration in cultures lacking antigen, and is considered positive if ≥ 3 (shown in bold type).
†
P < 0·05 when diabetic TB compared with non-diabetic TB patients;
‡
P < 0·05 when diabetic TB compared with healthy subjects. BCG, bacille Calmette–Guérin; MT-CF, Mycobacterium tuberculosis culture filtrate; MT-CW, M. tuberculosis cell walls.