Table 7.
T helper type 1 (Th1) : Th2 type cytokine ratios in culture supernatants of peripheral blood mononuclear cells (PBMC) obtained from diabetic and non-diabetic tuberculosis (TB) patients and Mycobacterium bovis bacille Calmette–Guérin (BCG)-vaccinated healthy subjects in the presence of complex mycobacterial antigens and region of difference (RD)1pool.
| Cytokine ratios in culture supernatants of |
|||
|---|---|---|---|
| Antigen Th1 : Th2 cytokine | Diabetic TB patients | Non-diabetic TB patients | Healthy subjects |
| M. bovis BCG | |||
| IFN-γ : IL-4 | 31 | 337 | 123 |
| IFN-γ : IL-5 | 16·7 | 240 | 148 |
| IFN-γ : IL-10 | 3·7 | 13 | 21 |
| TNF-β : IL-4 | 1·3 | 5 | 3·3 |
| TNF-β : IL-5 | 0·7 | 3·3 | 4 |
| TNF-β : IL-10 | 0·2 | 0·2 | 0·6 |
| M. tuberculosis | |||
| IFN-γ : IL-4 | 27 | 231 | 152 |
| IFN-γ : IL-5 | 10·5 | 134 | 183† |
| IFN-γ : IL-10 | 2 | 6·6 | 3 |
| TNF-β : IL-4 | 0·7 | 5 | 2·3 |
| TNF-β : IL-5 | 0·3 | 3 | 2·8 |
| TNF-β : IL-10 | 0·1 | 0·2 | 0·1 |
| MT-CF | |||
| IFN-γ : IL-4 | 103 | 420* | 623† |
| IFN-γ : IL-5 | 30 | 448* | 752† |
| IFN-γ : IL-10 | 14·5 | 33 | 399† |
| TNF-β : IL-4 | 10 | 10 | 11 |
| TNF-β : IL-5 | 3 | 11 | 13 |
| TNF-β : IL-10 | 1·4 | 0·8 | 7 |
| MT-CW | |||
| IFN-γ : IL-4 | 28 | 355 | 291 |
| IFN-γ : IL-5 | 12 | 189* | 352† |
| IFN-γ : IL-10 | 3·3 | 22 | 74 |
| TNF-β : IL-4 | 1·6 | 9 | 5 |
| TNF-β : IL-5 | 0·7 | 5 | 6 |
| TNF-β : IL-10 | 0·2 | 0·6 | 1·2 |
| RD1pool | |||
| IFN-γ : IL-4 | 17 | 63 | 25·6 |
| IFN-γ : IL-5 | 15·3 | 58 | 31 |
| IFN-γ : IL-10 | 8·6 | 58* | 23‡ |
| TNF-β : IL-4 | 1·1 | 2·2 | 0·5 |
| TNF-β : IL-5 | 1 | 2 | 0·6 |
| TNF-β : IL-10 | 0·6 | 2 | 0·5 |
*
P < 0·05 when diabetic TB compared with non-diabetic TB patients;
†
P < 0·05 when diabetic TB compared with healthy subjects.
‡
P < 0·05 when non-diabetic TB compared with healthy subjects. IFN, interferon; IL, interleukin; TNF, tumour necrosis factor; MT-CF, Mycobacterium tuberculosis culture filtrate; MT-CW, M. tuberculosis cell walls.