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Sequences for primers used in quantitative polymerase chain reaction (Q-PCR).
| Transcript | Forward primer | Reverse primer | Amplicon |
|---|---|---|---|
| IgG1 | CATCTCCAAAGCCAAAGG | ATGTCGCTGGGATAGAAG | 126 bp |
| IgG2–4 | CATCTCCAAAGCCAAAGG | ATGTCGCTGGGGTAGAAG | 126 bp |
| IgM | CAGGGCACAGACGAACAC | CGGCAATCACTGGAAGAGG | 85 bp |
| 18S rRNA | AGTCCCTGCCCTTTGTACACA | GATCCGAGGGCCTCACTAAAC | 68 bp |
Ig, immunoglobulin; bp, base pairs.
