Fig. 1.
Relative gene expression of chemokines and chemokine receptors in mouse kidney during the acute inflammation phase of anti-myeloperoxidase (MPO) immunoglobulin (Ig)G-induced necrotizing crescentic glomerulonephritis (NCGN). (a–e) Relative mRNA levels of (a) CC-chemokines, (b) CC-chemokine receptors, (c) CXC-chemokines, (d) CXC-chemokine receptors and (e) CX3C- and XC-chemokines and their receptors in kidneys from healthy control mice (white bars), mice that received lipopolysaccharide (LPS) only (grey bars) and mice that received anti-MPO IgG and LPS (black bars) 1 day after administration. Bars represent mean ± standard deviation (s.d.). *P < 0·05 versus control mice; #P < 0·05 versus LPS-treated mice. (f–j) Relative mRNA levels in laser-microdissected glomeruli (striped bars) and tubulo-interstitial areas (dotted bars) from mice that received anti-MPO IgG and LPS 1 day after administration. (f) CC-chemokines, (g) CC-chemokine receptors, (h) CXC-chemokines, (i) CXC-chemokine receptors and (j) CX3C- and XC-chemokines and their receptors. Bars represent mean ± s.d. *P < 0·05; n.d.: not detected; n.t.: not tested – CXCR4 could not be analysed due to insufficient amplification reactions.