Figure 4. Transcription start sites (TSSs) of the poxB and acs genes were determined using a modified 5′RACE methodology, as described in materials and methods.

The cDNAs in the bands were extracted and their nucleotide sequences determined to their 5′ends to allow TSSs determinations. The nucleotide sequences of the different cDNA, are presented in figure S1 and table 1. The consensus nucleotide sequence of the −10 region for the σ³⁸ sigma subunit (8 nucleotides framed in the figures) described by Weber et al [5], was utilized for detecting possible σ³⁸ recognition sites and a 10 nucleotides −10 σ⁷⁰ consensus sequence was utilized for detecting possible σ⁷⁰ binding sites. Numbers in parenthesis indicate references in which previously reported TSSs have been described: Wise et al [57], Chang et al [58], Kumari et al [60], and Beatty et al [61].