Figure 5. This figure shows the TSSs determined for the pfkA gene in an independent global transcription initiation mapping experiment in which random primers were utilized instead of specific oligonucleotides.

As can be seen in this experiment in which more than 1500 TSSs were determined (Mendoza et al, submitted to PLoS ONE), there are four of these transcripts that initiated at the same nucleotide A (78 nucleotides from the initiation ATG codon). This nucleotide has been reported as the transcription initiation site for the pfkA gene [39]. In addition, transcription also occurred in several cases from another site that initiated with a T residue, (29, nucleotides from the ATG start codon). It has been shown that transcription from the pfkAP1-P2 promoter is responsible for the previously reported transcription event and, pfkAP3-P4 is responsible for a second event (figure 2c).