Figure 2.

Expression of mCry1 and mCry2 in mouse retina. In situ hybridization was performed with ³⁵S-labeled antisense RNA of the appropriate genes. (A) Dark-field microgram showing uniform expression of both mCry1 and mCry2 in the GCL and INL from macular (M) region to peripheral retina. Silver grains detected in the outer segment (OS) are due to absorption of light by residual pigment epithelium that remained in these retinal preparations. (Bar = 200 μm.) (B) Bright-field microgram. The mRNA locations of CRY1, CRY2, and opsin are compared. Note that mCry1 and mCry2 are expressed in the GCL and INL only. Arrows indicate clusters of ganglion cells that express Cry1 and Cry2. In contrast, opsin is highly expressed in the inner segment (IS) and the outer nuclear layer (ONL) and is not detected in GCL and INL. The other layers of the retina are also indicated as follows: IPL, inner plexiform layer; OPL, outer plexiform layer. (Bar = 30 μm.)