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. Author manuscript; available in PMC: 2010 Jan 17.
Published in final edited form as: Immunity. 2009 Jul 2;31(1):110–121. doi: 10.1016/j.immuni.2009.04.015

Figure 4. Activation of an IL-1α-mediated innate response to Ad does not depend on NALP3-inflammasome, MyD88, TRIF, or TRAF6.

Figure 4

(A) Mice were injected with Ad and mRNA levels for IL-1α, IL-1β, KC and MIP-2 were analyzed 30 min p.i. N=4. Except for the columns indicated by the star, no statistically significant differences between experimental groups and WT group were identified (P > 0.05). * - P < 0.01. C – mock-infected mice, treated with saline. AU – arbitrary units. (B) Analysis of proteins in spleens of mice 1 hour after Ad injection. N=4. Mock – negative control mice injected with saline. Pos-C are dots that show the manufacturer’s internal positive control samples on the membrane. (C) The amounts of pro-inflammatory cytokines and chemokines in the spleens of mice 1 hour after Ad injection. N=3. Mock – negative control mice injected with saline. n.s. – no statistically significant difference were identified between WT and gene knockout animals (P > 0.05). (D) IL-1α, MIP-2 and KC mRNA levels in spleens of mice 30 min after Ad injection. N=4. * - P < 0.01. C – mock-infected mice, treated with saline. n.s. - not statistically significant.