Table 1. Relative frequency of primary Vpr allelesa.
Samples | Vpr alleles | Number of clones/total number of sequenced clones (%) |
Sample 1986 | LTNP-86 | 40/40 (100) |
Sample 1996 | LTNP-96 | 40/40 (100) |
Sample 2004 | LTNP-04-1 | 23/40 (57.5) |
LTNP-04-2 | 6/40 (15) | |
LTNP-04-3 | 3/40 (7.5) | |
LTNP-04-4 | 3/40 (7.5) | |
LTNP-04-5 | 5/40 (12.5) | |
Sample PR | PR1 | 18/42 (43) |
PR2 | 18/42 (43) | |
PR3 | 6/42 (14) |
vpr genes were amplified by PCR from each PBMC sample (indicated by blue arrows in Fig. 1A), cloned into a shuttle plasmid, and DNA sequences from at least 40 independent clones were determined from each sample.
samples 1986, 1996 and 2004 are from the LTNP patient (5071), and sample PR is from the progressor patient (1200).
Vpr alleles correspond to the primary amino acid sequences reported in Fig. 1B.