Figure 3. The basal activity of MEK/ERK signaling was sufficient for MEK inhibitors-enhanced HCV replication.

HCV replicon cells were cultured in a 24- or 6-well plate in the absence or presence of serum for 24 hr and treated with either MEK inhibitors or DMSO for 24 hr. A. Lysates were subjected to the luciferase reporter gene activity assay and the data were expressed as mean±SEM of triplicates. B. Total RNA was assayed for plus-strand HCV RNA by RPA and the data were quantitated as above and is representative of three independent experiments.