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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jul 23;1790(10):1179–1190. doi: 10.1016/j.bbagen.2009.07.012

Figure 3. Overexpression of 190 results in diminished stress fiber and focal adhesion formation.

Figure 3

Figure 3

Figure 3

Figure 3

PAEC were transfected cDNA encoding with GFP (panels a-d), GFP-conjugated wild type (wt) p190 (panels e-h), or GFP-conjugated dominant negative (dn) p190 (panels i-l). At 48h post-transfection, endothelial cells were fixed and immunofluorescently stained for vinculin using Texas Red-conjugated secondary antibodies directed against vinculin antibodies (panels d, h, l) or filamentous actin using Texas Red-conjugated phalloidin (panels c, g, k). Nuclei were counterstained using DAPI. Endothelial cells were visualized via fluorescence microscopy. Arrows indicate representative transfected endothelial cells. Images are representative of three independent experiments and 6−9 images were obtained for each experiment. Scale bars = 20μm. Panel m, number of focal adhesions per endothelial cell was quantitated as the number of immunofluorescently stained vinculin structures relative to total number of transfected endothelial cells for each construct. Data is presented as the mean ± SEM. n = 3; * p<0.05 vs. GFP or GFP-DN p190.