PAEC were transfected with cDNA encoding GFP, GFP-conjugated wild type (WT) p190, or GFP-conjugated dominant negative (DN) p190. At 48h posttransfection, GFP-positive cells were purified from untransfected endothelial cells using FACs analysis (panel a). Purified endothelial cells overexpressing the indicated construct were harvested and RhoA activity determined. Densitometric analysis was used to quantitate the level of active RhoA relative to total RhoA. Data is presented as mean ± SEM. n = 3; * p <0.05 vs. GFP or GFP-DN p190. p = 0.104, GFP vs GFP-DN p190. Panel b, in parallel, lysates were resolved by SDS-PAGE, transferred to membrane, and immunoblotted for GFP to demonstrate protein overexpression.