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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jul 23;1790(10):1179–1190. doi: 10.1016/j.bbagen.2009.07.012

Figure 7. Suppression of p190 and p190-B individually or concurrently does not attenuate increased permeability upon PKCδ inhibition.

Figure 7

Figure 7

Figure 7

Equivalent numbers of LMVEC were transfected with p190 siRNA (panel a), p190-B siRNA (panel b), both p190 and p190-B siRNA (panel c). In parallel, in each panel equivalent numbers of LMVEC were transfected with non-silencing (ΝΣ) σιΡΝΑ. Αλλ τρανσϕεχτεδ ενδοτηελιαλ χελλσ ωερε πλατεδ οντο χολλαγεν χοατεδ ΕΧΙΣ αρραψσ. Ατ 96η ποσττρανσϕεχτιον, ενδοτηελιαλ χελλσ ωερε τρεατεδ ωιτη ϖεηιχλε (ΔΜΣΟ) ορ 5μM rottlerin and changes in resistance across endothelial monolayer were measured. Values are means ± SE of the normalized resistance. Arrow indicates time of addition of vehicle or rottlerin. n = 3−7; *p<0.05 vs. vehicle. Insert: endothelial cells were collected following each experiment and equivalent amounts of lysates were resolved via SDS-PAGE and immunoblotted for p190 to confirm protein suppression. Membranes were stripped and reprobed for actin to confirmed equal protein loading.