Figure 3.

RO‐3306 cooperated with Nutlin‐3 in activating Bax and inducing mitochondrial apoptosis. AML cells were treated for 8 h with 5 µM RO‐3306 or 2.5 µM Nutlin‐3 (5 µM in OCI‐AML‐3). (a) The percentages of Bax in its active conformation were determined by staining with the active conformation‐specific anti‐Bax antibody YTH‐6A7 (shaded histogram) or a corresponding isotype control (open histogram). To block caspase activation‐mediated conformational change of Bax, cells were preincubated for 1 h with 50 µM Z‐VAD‐FMK. (b) Δψ_m was assessed by flow cytometry. The results are expressed as the mean ± SD of triplicate measurements. *Significance at P < 0.01.