Fig. 7.
Separation of proteins using monolithic capillary grafted with N,N-dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl) ammonium betaine. (Reprinted with permission from ref. [119]. Copyright 2004 Elsevier) Conditions: 50 µm capillary column total length 34.5 cm, monolith 8.5cm, 1 min grafting; mobile phase 5 mmol/L phosphate buffer (pH 7.0)/acetonitrile (20+80); voltage −15 kV; overpressure in both vials 0.8 MPa; temperature 60 °C. Peaks (1) cytochrome c, (2) holotransferrin, (3) ribonuclease a, (4) myoglobin.