Figure 1. mRNA Translation Associated Changes Upon DR.

(A) Polysomal distribution of mRNAs of young adult male flies on 4% YE and 0.25% YE for 6 days showing the individual ribosomal subunits and the polysome peaks, normalized to body weight of flies. RNA was prepared from the low (Lo) and high (Hi) translation fractions for microarray analysis performed in triplicate.

(B) Levels of ³⁵S-methionine incorporation in 7 day old control flies normalized to protein content on 4% YE or 0.25% YE. (±SEM) (N=3) (*p<0.05, ** p<0.01).

(C) 4E-BP protein is induced on 0.25% YE (DR) as measured by western blot using 30μg of protein probed with a polyclonal anti-d4E-BP (Miron et al., 2001). This induction is not dependent on dFOXO, as dFOXO null mutant flies show a normal response. β-tubulin levels were measured as a loading control. The blot is representative of three replicates.

(D) Functional grouping of differentially translated genes according to GO terminology using DAVID analysis software.