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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Nat Med. 2009 Sep 6;15(10):1163–1169. doi: 10.1038/nm.2009

Figure 4.

Figure 4

Integrin αvβ3-induces anchorage-independent survival with no affect on survival of adherent cells. (a–c) Expression of αvβ3 in FG cells enhanced colony number in soft agar (a,b) while knock-down of β3 in Panc-1 cells (β3 sh) reduced colony formation compared to cells expressing a non-silencing control (n-s) (c). n=3 independent experiments. *P<0.05. (d) FG and FG-β3 cells were maintained in suspension culture for 24 and 48 h prior to trypan blue staining and counting viable and non-viable cells. Compared to FG control cells, suspension cultures of FG-β3 cells exhibit significantly less cell death. n=4 independent experiments. *P<0.05. (e,f) Ligation of αvβ3 to fibronectin failed to protect FG-β3 cells from apoptosis initiated by either gemcitabine (e) or anti-Fas antibody (CH11) (f). Representative experiments are shown.