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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nat Med. 2009 Sep 13;15(10):1219–1223. doi: 10.1038/nm.1971

Figure 1.

Figure 1

Principles of in vivo multiparametric imaging with optical frequency domain imaging (OFDI). (a) An optical beam is focused into the tissue. The light reflected across all depths is combined with a reference beam and the interference signal is recorded as a function of light wavelength from 1,220 nm to 1,360 nm. The amplitude and phase of the reflected light as a function of wavelength is used to localize the reflected signal as a function of depth. At a given depth, the amplitude and phase of the reflected signal as a function of time is used to derive the optical scattering properties and thereby the tissue structure and function.(b) The depth-projected vasculature within the first 2 mm of mouse brain bearing a xenotransplanted U87 human glioblastoma multiforme tumor imaged with OFDI. Depth is denoted by color: yellow (superficial) to red (deep). Scale bar, 500 μm.