Fig. 4.

Pure populations of epithelial cells were obtained from histologically normal buccal mucosa (lanes 6–10) or tissues that exhibit features of oral lichen planus (OLP) (lanes 1–5) by laser capture microdissection. (a) Expression of CD14 mRNA [758 base pairs (bp)], Toll-like receptor (TLR)-2 mRNA (498 bp) and TLR-4 mRNA (398 bp). The first lane (indicated) in the top three panels represent respective mRNA expression in Thp-1 cells. Expression of SPRR2a (110 bp) abundant in oral epithelium was amplified as control. (b) Quantitation of the band intensity was performed by densitometry. Data are presented as average intensity ± standard error. *P = 0·05.