Fig. 6.
Minor contribution of interferon regulatory factor-3 (IRF-3) on poly I:C-stimulated induction of intercellular adhesion molecule-1 (ICAM-1). HT-29 cells, 2 × 105, were plated on a 24-well plate and transfected with siRNA against IRF-3 or control siRNA for 6 h. The cells were cultured for a further 42 h with 10% fetal calf serum–Dulbecco's modified Eagle's medium. (a) At this time-point, the cell lysates were prepared and 40 µg of protein was subjected to Western blotting. The representative data of three experiments were shown. (b) After transfection, the cells were stimulated with poly I:C for 24 h and the culture supernatants were collected and subjected to enzyme-linked immunosorbent assay. The means of four different experiments were expressed. *P < 0·05 for the comparison of siRNA against IRF-3 transfection plus and minus conditions.