Fig. 5.

C3 deposition on apoptotic Jurkat cells after incubation with complement-deficient sera at different concentrations. (a) C1q-deficient serum (C1qD serum; patient C1qD-2); (b) C4-deficient serum (C4D serum); (c) C2-deficient serum (C2D serum; patient C2D-1 and C2D-2); (d) C3-deficient serum (C3D serum). C3 deposition was measured by flow cytometry using antibodies to C3d, and deposition of C3 is given as mean fluorescence intensity (MFI).Early apoptotic and late apoptotic cells are given as early AC and late AC respectively.