Fig. 3.

Fractalkine (10 nM) was added to osteoarthritis (OA) fibroblasts for the times indicated and cell lysates were generated. Protein (20 µg/lane) was separated using polyacrylamide gel electrophoresis and blotted using standard Western techniques. Phosphorylated c-Jun N-terminal kinase (a) and p38 (b) were compared with the total amount of their respective proteins to assure equal loading. Bars represent normalized band intensity in arbitrary units. Blots are representative of three different experiments performed using OA fibroblasts from three different patients.