Fig. 7.

(a) Frequency of forkhead box P3 (FoxP3⁺) cells in splenic CD4⁺ T cells from MIH-2-bearing mice or non-tumour-bearing mice immunized excessively with CYP2J peptide. Splenocytes were collected from each group of mice, and CD4⁺ T cells were obtained by magnetic sorting. The frequency of FoxP3⁺ cells in CD4⁺ T cells was examined with flow cytometry. (i) Non-MIH-2-bearing mice treated with phosphate-buffered saline (PBS); (ii) non-MIH-2-bearing mice immunized with CYP2J peptide 7 times; (iii) MIH-2-bearing mice treated with PBS; (iv) MIH-2-bearing mice immunized seven times with CYP2J peptide. (b) Frequency of CD11b⁺Gr-1⁺ cells in splenocytes from MIH-2-bearing mice or non-MIH-2-bearing mice immunized excessively with CYP2J peptide. Splenocytes were collected from each group of mice, stained with fluorescein isothiocyanate-conjugated monoclonal antibodies (mAbs) to Gr-1 and with phycoerythrin-conjugated mAbs to CD11b, and analysed with two-colour flow cytometry. (i) Non-MIH-2-bearing mice treated with PBS; (ii) non-MIH-2-bearing mice immunized seven times with CYP2J peptide; (iii) MIH-2-bearing mice treated with PBS; (iv) MIH-2-bearing mice immunized seven times with CYP2J peptide.