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Reproducibility of single-cycle HIV-1 infectivity assay in functional screening. Two sets of scFv were made and tested in separate infectivity assays 2A and 2B. Results of 11 clones are shown from each of the two runs. S1 to S4 indicate the dilution of each sample. The columns indicate the amount of HIV-1 that enters the P4/R5 cells. Clones D5 and D7 show inhibition at all 4 dilutions. Clone D9 shows the least amount of viral inhibition.
