Skip to main content
. 2009 Sep-Oct;1(5):475–480. doi: 10.4161/mabs.1.5.9580

Figure 2.

Figure 2

Binding of IGF-I and IGF-II to recombinant IGF-IR ectodomain. Two micrograms per milliliter recombinant IGF-IR ectodomain were coated on 96-well Maxisorp plate. After blocking the plates with 3% BSA in PBS, two-fold serially diluted IGF-I and IGF-II premature form with a starting concentration of 5 µg/ml were added to the plates. Bound IGF-I were detected with goat anti-IGF-I pAb (1:1,000) and HRP conjugates to anti-goat pAb (1:1,000). Bound IGF-II premature form were detected with HRP conjugates to penta-his tag antibody (1:1,000). Optical density at 405 nm was measured 20 min after adding ABTS substrate.