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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Biochem Pharmacol. 2009 May 27;78(8):966–973. doi: 10.1016/j.bcp.2009.05.025

Fig. 4. Knockdown of Bax and Bak inhibit Bz-423-induced apoptosis.

Fig. 4

Ramos cells were transfected with siRNA for control (circles, solid line), Bax (squares, long dashes), Bak (diamonds, short dashes), or both Bax and Bak (triangles, dotted line) followed by treatment with Bz-423. (A) Superoxide production (1 h) was measured using DHE. (B) ΔΨm (6 h) was measured using DIOC6(3). * = P < 0.05 for Control vs. Bax or Bak or Bax/Bak; P < 0.05 also for Bax vs. Bak at [Bz-423] ≥ 5 µM. (C) Cell viability (24 h) was measured by PI exclusion. * = P < 0.05 for Control vs. Bax or Bak or Bax/Bak; P < 0.05 also for Bax vs. Bak at [Bz-423] ≥ 6 µM. (D) Apoptosis (24 h) was measured by identifying cells with subG0-DNA content. * = P < 0.05 for Control vs. Bax or Bak or Bax/Bak; P < 0.05 also for Bax vs. Bak at [Bz-423] ≥ 5 µM.