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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Biochem Pharmacol. 2009 May 27;78(8):966–973. doi: 10.1016/j.bcp.2009.05.025

Fig. 6. Knockdown of BH3-only proteins inhibits Bz-423-induced cell death.

Fig. 6

(A) Ramos cells were transfected with siRNA for the indicated BH3-only proteins followed by preparation of total cell lysates and determination of Bad, Bim, Bik, and Puma by immunoblot. All represents lysates from cells transfected with siRNA for Bad, Bim, Bik, and Puma. (B) Ramos cells were transfected with siRNA for control (circles, solid line), Bad (squares, long dashes), Bik (diamonds, short dashes), Bim (triangles, dotted line), Puma (inverted triangles, alternating long and short dashes), or All (open circles, solid line) followed by treatment with Bz-423. Cell viability (24 h) was determined by PI exclusion. ** = P < 0.001 for Control vs. All. In addition, P < 0.01 for Control vs. Bad at [Bz-423] ≥ 6 µM; P < 0.01 for Control vs. Bik at [Bz-423] ≥ 5 µM; P < 0.02 for Control vs. Bim at [Bz-423] ≥ 5 µM; P < 0.02 for Control vs. Puma at [Bz-423] ≥ 6 µM; P < 0.05 for All vs. Bad or Bik or Bim or Puma at all [Bz-423] ≥ 3 µM.