Figure 1. Activation of GFP-tdrd7 expression by DAZL.

(A) GFP-tdrd7 and DsRed mRNAs were co-injected with the mRNAs encoding Myc-tagged DAZL, DAZL F91A, DAZL RRM, or DAZL ΔDAZ mRNA, or without any dazl mRNA (mock) at the one-cell stage. GFP and DsRed expression were analyzed at 24 hr post-fertilization (hpf). Fold change of normalized GFP fluorescence relative to mock control is shown on the right. (B) In situ hybridization of the injected embryos at 80% epiboly with an antisense probe for GFP. GFP-tdrd7 and DsRed mRNAs were injected with or without the mRNA encoding Myc-DAZL. (C) Northern blotting of the injected GFP-tdrd7 and DsRed mRNAs. The ratio of GFP mRNA to DsRed mRNA from three independent experiments is shown.