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. 2009 Oct 16;4(10):e7480. doi: 10.1371/journal.pone.0007480

Figure 5. Effect of SNP rs365165, 3′region genotypes, on human SNCA-mRNA expression levels in human brains.

Figure 5

Individuals were genotyped for SNP rs365165. Three brain regions were analyzed: temporal cortex (A), midbrain including SN (B) and frontal cortex (C). In each brain region fold levels of human SNCA-mRNA were assayed by real-time RT-PCR using TaqMan technology and calculated relative to human SYP-mRNA reference control using the 2-ΔΔCt method. (A) Analysis of the temporal cortex showed that the protective genotype AA correlates with higher SNCA-mRNA levels than the GA and GG genotypes (P<0.05). (B) In the midbrain including SN, the AA and AG genotypes correlate with higher SNCA-mRNA levels compared with the GG risk genotype (P<0.05). (C) No correlations of SNP rs365165 genotypes with SNCA-mRNA levels were detected in the frontal cortex. For each genotype, the box plot represents the analysis performed using all brain samples available from the specific brain region, each of which was analyzed twice independently, each time in duplicate. The average values are presented in ‘X’. The box plot shows the median (horizontal line inside the box) and the 25th and 75th percentiles (horizontal borders of the box). The range between the 25th and 75th percentiles is the interquartile-range (IQR). The whiskers show the minimal and maximal values inside the main data body.