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. 2009 Aug 19;64A(11):1146–1153. doi: 10.1093/gerona/glp118

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Published by Oxford University Press on behalf of The Gerontological Society of America 2009.

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Figure 4. — Cellular fractionation and immunoblotting of aging mouse tissues. Kidney (A) and Liver (B) tissues were fractionated into cytoplasmic (c) and membrane (m) components before being run on sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Claudin-1 (Cldn1) and claudin-4 (Cldn4) expression patterns were observed. The lysates were also probed for glyceraldehyde 3-phosphate dehydrogenease (a cytoplasmic control) and E-cadherin (a membrane control). Claudin-1 is located predominantly in the membrane fraction and is increased in the kidney of older animals. Claudin-4, on the other hand, in mostly cytoplasmic and is decreased in both the kidney and liver of older mice.