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. 2009 Oct 15;4(10):e7470. doi: 10.1371/journal.pone.0007470

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Venkatachari et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immature and mature MDDC were cocultured with HIV-1^wt-EGFP virus infected PBL at a ratio of 2∶9∶1 (DC:uninfected PBL:infected PBL) either directly or separated by a transwell. Post coculture (12 hours) cells were stained for DC-SIGN, and analyzed by flow cytometry. Gating was extended to include lymphocytes and doublet differentiation was applied. (B) Cell contact dependent productive infection of immature and mature DC by lymphocyte associated HIV-1 virus in multiple donors (n = 8). (C) Time kinetics of productive infection in DC mediated by T cell associated virus. The figure is representative of data acquired from multiple donors (n = 5). Error bars indicate S.D. of the results obtained from triplicate wells from a single donor.