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. Author manuscript; available in PMC: 2009 Oct 9.
Published in final edited form as: Oncogene. 2006 Apr 20;25(17):2452–2467. doi: 10.1038/sj.onc.1209287

Figure 7.

Figure 7

ADAMTS-1NTF and ADAMTS-1CTF inhibit activation of Erk1/2 kinases induced by soluble AR and HB-EGF. Soluble AR (5 ng/ml), HB-EGF (4 ng/ml), or bFGF (15 ng/ml) was applied to serum-starved MCF-10A cells for 25 min in the absence (lanes 3 and 4) or presence of 400 ng of purified full-length ADAMTS-1 (lanes 5 and 6), ADAMTS-1E/Q (lanes 7 and 8), ADAMTS-1NTF (lanes 9 and 10), or ADAMTS-1CTF (lanes 11 and 12). Serum-free medium alone was applied to cells in lanes 1 and 2. The cells were lysed and equal amounts of protein were analysed by Western blotting with anti-phospho-Erk1/2 antibody to detect phosphor-Erk1/2 (upper panels, a–c) or with anti-Erk antibody to detect the total amount of Erk1/2 (bottom panels, a–c).