Fig. 4.
Effect of 36 h fasting on CYP2E1 activity, MDA formation, NOS activity, H2O2 production, and total and mitochondrial GSH. Equal amounts of whole liver lysates or mitochondrial proteins from different groups were used to determine (A) CYP2E1 activity by measuring the rate of PNP oxidation to p-nitrocatechol, (B) hepatic malondialdehyde (MDA), (C) NOS activity, (D) H2O2 production rates, (E) total GSH and (F) mitochondrial GSH levels, as described in Materials and Methods. Data are expressed as mean ± S.E.M. of 3 mice per group. &Significantly different from fed wild-type; *significantly different from corresponding wild-type group; #significantly different from fed Ppara-null mice.